Roles of RNA Secondary Structure in Dengue Virus C Gene and Capsid Protein in Viral Replication 登革病毒C基因RNA二级结构及编码蛋白在病毒复制中的作用
Inhibitory Effect of Small Interfering RNA Targeting X Gene on Hepatitis B Virus Replication 体外针对X基因的小干扰RNA的抗乙型肝炎病毒效应研究
The primary function of the influenza virus nucleoprotein ( NP) is to encapsidate the virus genome for the purposes of RNA transcription, replication and packing. 流感病毒核蛋白(np)主要功能是使病毒基因组衣壳化,以便RNA转录、制和病毒子装配。
Effect of mutations in dengue virus C gene on viral RNA replication 登革病毒C基因突变对病毒RNA复制的影响
Initiation of Genomic RNA Replication by a Recombinant Classical Swine Fever Virus RNA-dependent RNA Polymerase 猪瘟病毒RNA依赖的RNA聚合酶起始基因组RNA复制的分子机制研究
In addition, these findings provide some clues to further work. The research on viral or cellular factors involved in the RNA synthesis from positive-strand RNA, as well as the features of negative-strand RNA template will lead to the better understanding of HCV replication. 同时,本实验的结果也为进一步研究病毒或其它细胞因子参与以正链RNA为模板进行的RNA合成,以及有关负链RNA模板特性的研究奠定了基础。
Objective To investigate the effect of dual-target antisense RNA of hepatitis B virus ( HBV) targeted to X and P region on replication and expression of HBV gene in HBV transgenic mice. 目的探讨针对乙型肝炎病毒(HBV)x、p双靶区反义RNA对乙型肝炎病毒转基因小鼠HBV复制和表达的影响。
The preliminary results showed that the conservation of nucleotide sequence in the loop region and the base-pairing in the stem of the stem-loop structures were necessary to keep the correct RNA folding essential for the virus replication. 结果表明,茎-环结构中环部核苷酸种类的保守性、以及茎部的碱基配对程度对于维持病毒正常复制所必需的RNA结构至关重要。
The genomes of viruses in family Geminiviridae are single stranded DNA, and their replication strategies are distinctly different from that of RNA viruses. The geminiviruses use the replication machinery of the host and hence have been presumed to have a high fidelity of replication. 双生病毒(geminiviruses)是一类单链DNA病毒,其复制方式有别于植物RNA病毒,他们是利用寄主植物的DNA复制酶复制,理论上其忠实性远高于RNA病毒。
Effect of Astragalus membranaceus on Ca  ̄( 2+) Influx and Coxsackie Virus B_3 RNA Replication in Cultured Neonatal Rat Heart Cells 黄芪对柯萨奇B3病毒感染培养大鼠心肌细胞Ca~(2+)内流及该病毒RNA复制
Conclusion Dual-target antisense RNA targeting the X and P regions in the genome of HBV inhibits the replication and expression of HBV, significantly stronger than single-target antisense-RNA. 结论HBVx、P双靶区反义RNA对乙型肝炎病毒转基因鼠有显著抗HBV作用,且在作用效率和作用时间上优于单靶区反义RNA。
Both DNA and RNA can act as genetic materials. The molecular mechanism of DNA replication has been lucidly illuminated, however, the RNA replication has not been fully understood. DNA和RNA都是遗传物质,人们对于DNA的复制机制已经有了比较透彻的了解,但对RNA的复制还不清楚。
Function of RNA-dependent RNA Polymerase of Influenza A Virus on the Regulation of Viral Genome Replication and Transcription A型流感病毒RNA聚合酶及其对基因组复制和转录的调控作用
HCV helicase is responsible for unwinding of RNA genome and play a key role in the replication of HCV genome. The HCV helicase gene was amplified by RT-PCR from the HCV positive serum. 解旋酶(helicase)在HCV基因组复制中负责RNA的解链,在复制中起着关键的作用。
Conclusions We have established a HCV RNA polymerase expression system in Hub-7 cells that can be used to study the mechanism of HCV replication and to test gene therapy of hepatitis C. 结论我们在Hub-7细胞中建立了HCV-RNA多聚酶表达系统,它有助于研究HCV复制机制和为基因治疗丙型肝炎打下基础。
CONCLUSION: RNA interference can inhibit HBV gene expression and virus replication. 结论:RNA干扰能抑制HBV基因表达及病毒复制,并且RNA干扰可能为HBV的治疗带来巨大的变化。
RNA silencing occurs in plants is conceived as a natural antiviral defense system that is activated as a response to double? stranded RNA ( dsRNA) formed during virus replication. 植物RNA沉默(RNAsilencing)是植物本身固有的一种抗病毒防御系统,是对病毒在复制过程中形成双链RNA(dsRNA)的特殊反应。
RNA interference inhibits hepatitis B virus gene expression and replication in HepG_2-N10 cells RNA干扰抑制HepG2-N10细胞系中HBV基因表达及复制
RNA dependent RNA polymerase is the major catalyst for transcription/ replication. 依赖于RNA的RNA聚合酶是RNA病毒转录/复制的主要催化剂。
The RNA product of the replication system in cells was quantified by strand-specific real-time RT-PCR. The results suggested that the RdRp could dramatically improve the synthesis of RNA. 利用RNA细胞内复制体系和荧光定量RT-PCR技术,证明3D基因表达产物在细胞内可促进口蹄疫病毒基因组的复制。
RNA Interference Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus 利用RNA干扰机制抑制猪繁殖与呼吸综合征病毒增殖
Reverse genetics, as a novel technique, has played an important role in study of structure, function of genome of negative-strand RNA virus and exploration of its replication, transcription and development of vaccine. 反向遗传技术是一种新的分子生物学技术,它在深入研究负链RNA病毒基因组结构和功能,探寻其基因组复制、转录和研发新型基因工程疫苗上发挥着重要的作用。
HCV RNA is a reliable marker of replication. ALT level reflects replication status and inflammation state of the liver. HCVRNA是反映HCV复制的可靠指标,结合ALT的结果可帮助临床了解HCV在体内的复制状况及肝脏的炎症状态。
Because most RNA virus have the characteristics of selective replication in tumor cells, NDV is interesting to be a new viral carrier in tumor gene therapy in recent years. 由于多数RNA病毒具有天然地在肿瘤细胞选择性复制的特点,近年来用这类病毒作为新型运载工具在肿瘤基因治疗方面引起了人们的关注。
The length of telomere gradually shortened as the number of cell divisions increase, and telomerase which used its RNA template to synthesis telomere fragments can compensate for deletions caused by replication. 端粒长度随着细胞分裂次数的增加而逐渐缩短,能利用自身RNA模板合成端粒片段的端粒酶可以弥补复制时造成的片段缺失。
Positive-strand RNA virus reverse genetics system is an useful tool in viral replication, pathogenesis research, screening antiviral drugs and new vaccines, vector-oriented areas such as the preparation. 而正链RNA病毒的反向遗传操作系统在病毒复制、致病机制研究,抗病毒药物筛选和新型疫苗、导向载体制备等方面是一个极其有用的工具。
In this study, we provide the first direct evidence that a liver-specific microRNA, miR-122, binds to a highly conserved HBV pregenomic RNA sequence via base-pairing interactions and inhibits HBV gene expression and replication. 在这项研究中,我们首次提供了一个肝脏特异的微小RNA,miR-122,通过碱基配对相互作用方式与一个高度保守的HBV前基因组RNA序列结合,抑制HBV基因表达和复制的直接证据。
It was discussed that the RNA was recombined from abortion replication or linked by two recombinant fragments through the recombinant site directly. 分析推测重组系由RNA流产性复制引起或重组的两个片段通过重组点联结形成。
Other than C protein itself, the gene of C protein also plays important roles in viral RNA replication and translation. 除C蛋白本身外,C基因在病毒RNA复制和翻译过程中也发挥了重要作用。